optimization of the expression of reteplase in escherichia coli

نویسندگان

h mir mohammad sadeghi

m rabbani

e rismani

f moazen

چکیده

reteplase is a segment of tissue plasminogen activator used for the removal of thrombi in blood vessels. in the present study the cloned reteplase gene was used for its expression in competent e. coli. the  recombinant plasmid, pet15b/reteplase (rpet-bl21), was transformed into competent e.coli strain bl21 (de3) cells. overnight culture of the transformed bacteria was induced by the addition of isopropylthio-ß-dgalactoside (iptg) to the final concentrations of 0.25, 0.5, 1 and 1.5 mm. also, the effects of different temperatures(25, 30, 37 and 39°c), shaking speeds (100, 170 and 190 rpm), and various glucose concentrations (0.25, 0.5, 0.75 and 1 mm) on the expression of reteplase were examined. samples were analyzed by sds-page. maximum amount of protein production was obtained by the addition of 1 mm iptg at 37°c, 100 rpm of shaking speed in the absence of glucose.

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optimization of the expression of reteplase in escherichia coli top10 using arabinose promoter

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expression and activity evaluation of reteplase in escherichia coli top10

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Optimization of the Expression of Reteplase in Escherichia coli TOP10 Using Arabinose Promoter

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cloning and expression of functional reteplase in escherichia coli top10

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عنوان ژورنال:
research in pharmaceutical sciences

جلد ۶، شماره ۲، صفحات ۸۷-۰

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